Optimizing Real-Time PCR for Reliable Gene Expression Studies

Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across different biological conditions and are a crucial element to many research studies today. In this webinar Dr. John Pfeiffer breaks down what it really takes to get accurate, reproducible real-time PCR gene expression data. You’ll learn why many experiments produce results that are difficult to interpret unless you understand assay specificity, define the dynamic range of your assay system, and run the right ongoing controls. Dr. Pfeiffer compares TaqMan probe-based and SYBR Green approaches, shows real examples, and gives simple, testable QC steps you can implement right away.

Learning Objectives:

• Learn how to better understand your qPCR assay specificity and how to confidently exclude non-target amplification

• Discover how to determine the dynamic range of your qPCR gene expression workflow to avoid data interpretation errors

• Enable more reliable gene expression data by implementing practical controls and QC strategies

For Research Use Only. Not for use in diagnostic procedures. © 2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license.