Accelerating Chemical Cross-Linking Optimization with Denaturing Mass Photometry

Chemical cross-linking (XL) is widely used to investigate protein–protein interactions (PPIs), including transient or low abundance subcomplexes in mass spectrometry workflows. However, identifying suitable XL reagents and optimizing reaction conditions can be slow and labor intensive, and standard analytical techniques such as SDS-PAGE often lack the resolution needed to accurately characterize complex assemblies.

In this webinar Dr Oscar Hernandez Alba, Research Scientist from the BioOrganic Mass Spectrometry Laboratory, CNRS, will compare denaturing mass photometry (dMP) with SDS-PAGE for screening XL conditions, demonstrating key advantages including: rapid screening of 20 XL conditions in one hour (3mins/triplicate), 20–100x lower sample consumption and single molecule sensitivity. By the end of the session, participants will have a clear understanding of how dMP enhances structural biology workflows, enabling comprehensive characterization of PPIs and macromolecular assemblies, including intermediate states prior to downstream analysis.

By attending this webinar, you will discover how mass photometry:

• Enables rapid, sensitive analysis ideal for high throughput XL condition screening before resource intensive experiments like mass spectrometry. 
• Is simple to use and highly accessible.
• Is compatible with both native and denaturing conditions.