UVA-excited dyes on qTOWER iris qPCR platforms enable sensitive thermal shift assays for challenging hydrophobic and membrane proteins.
Understanding protein stability is essential for drug discovery, protein research, and assay optimization. However, hydrophobic and membrane-associated proteins are challenging to analyze using conventional dye-based thermal shift assays, as visible-light dyes often produce high background signals and poor signal-to-noise ratios.
This application note shows how UVA-excited dyes such as CPM and 1,8-ANS, combined with the UV-ready qTOWER iris real-time PCR cycler, enable sensitive thermal shift assays and extend TSA workflows on qPCR instrumentation to challenging protein samples.
Inside this application note, you’ll learn:
Why conventional TSA dyes (e.g. SYPRO® Orange) are limiting for hydrophobic and membrane proteins
How CPM and 1,8-ANS detect thermal unfolding and melting temperatures (Tm) using UVA-excited fluorescence
How thermal shift assays are performed on qTOWER iris and qTOWER iris 384 with UV excitation (375 ± 15 nm) and optimized detection
Which key parameters were evaluated, including pH, salt composition, and reaction volume, in 96- and 384-well formats
How UVA-based TSA workflows support protein stability profiling and condition screening of challenging samples
Whether you work in drug discovery, membrane protein biophysics, or high‑throughput screening, this application note demonstrates how UVA‑based TSA workflows on a qPCR cycler use dyes suited for hydrophobic and membrane‑associated proteins to assess protein thermal stability.
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Analytik Jena is a provider of instruments and products in the areas of analytical measuring technology and life science. Its portfolio includes the most modern analytical technology and complete systems for bioanalytical applications in the life science area.
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